Caffeinated instant coffee prevents an increase in exercise-mediated superoxide anion production in rat peritoneal neutrophils

Elisson  Meireles Lamim Jerônimo; André Luiz Machado Viana; Miriam das Dores Mendes Fonseca; Marilene Lopes Ângelo; Bruno Cesar Correa Salles; Stella Maris da Silveira Duarte; Sônia Aparecida Figueiredo; Maria Rita Rodrigues; Fernanda Borges de Araujo Paula

Elisson Meireles Lamim Jerônimo Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil
André Luiz Machado Viana Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil
Miriam das Dores Mendes Fonseca Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil
Marilene Lopes Ângelo Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil
Bruno Cesar Correa Salles Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil
Stella Maris da Silveira Duarte Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil https://orcid.org/0000-0002-6276-3420
Sônia Aparecida Figueiredo Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil https://orcid.org/0000-0003-2549-4273
Maria Rita Rodrigues Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil https://orcid.org/0000-0003-0940-002X
Fernanda Borges de Araujo Paula Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brasil https://orcid.org/0000-0003-3077-3023

Keywords: Coffee, NADPH oxidase, Oxidative burst, Caffeine, Antioxidant, Exercise

Abstract

The present study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8% w/v) by adult male Wistar rats submitted to high-intensity exercises. The parameters used in the evaluation were the determination of the activities of NADPH oxidase, myeloperoxidase and other antioxidant enzymes present in neutrophils of rats. It was observed that exercise-induced superoxide anion production depends on the NADPH oxidase activity (estimated by the cytochrome C reduction test) in peritoneal neutrophils (p < 0.05). The intake of caffeinated and decaffeinated instant coffee beverages and of a caffeine solution to 1.67% did not induced changes in myeloperoxidase, superoxide dismutase and glutathione peroxidase activities in the peritoneal neutrophils of rats after exercise (p < 0.05). From the obtained results, it was concluded that moderate intake of caffeinated instant coffee (equivalent to a daily human consumption of 4 50-mL cups of coffee) may have beneficial effects on health, contributing to a reduction in superoxide anion generation triggered in neutrophils after high-intensity exercise.